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Gen-Probe 10-Q 2011 Exhibit 32.1
CERTIFICATION PURSUANT TO
18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 Pursuant to the requirement set forth in Rule 13a-14(b) of the Securities Exchange Act of
1934, as amended (the Exchange Act), and Section 1350 of Chapter 63 of Title 18 of the United
States Code (18 U.S.C. § 1350), Carl W. Hull, President and Chief Executive Officer of Gen-Probe
Incorporated (the Company), hereby certifies that, to the best of his knowledge:
IN WITNESS WHEREOF, the undersigned has set his hands hereto as of May 4, 2011.
This certification accompanies the Form 10-Q to which it relates, is not deemed filed with the
Securities and Exchange Commission and is not to be incorporated by reference into any filing of
Gen-Probe Incorporated under the Securities Act of 1933, as amended, or the Securities Exchange Act
of 1934, as amended (whether made before or after the date of the Form 10-Q), irrespective of any
general incorporation language contained in such filing.
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